Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. 00. Turn on Plate Reader and wait for it to complete its start-up routine. PURPOSE. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. For Raman excitation, a continuous-wave (CW) laser at 532 nm (Verdi V-18, Coherent) was used. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. 0 out of 5 $ 8,495. We would like to show you a description here but the site won’t allow us. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. Find our technical support information along with contact details. 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). use as little as 2 µL samples with 24- or 64-well plates. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. –Min. The former have been the traditional domain of plate readers and great strides have been. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Reliable performance with a proven track record. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 96-384. Figure 1. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Read one sample or up to 384. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. x. #S1207. Two high-energy xenon flash lamps: Reading capabilities. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 1. Therefore, it is recommended that the pipettor height to be set to correspond to the fluid volume in the cell plate prior to compound addition. 384-well TC plate, Poly-d-Lysine, Flat Clear. The plate reader can measure multiple readouts, including UV and. Plus 384. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Absorbance cuvette port. Outer wells are aligned for proper viewing with FLIPR. Artel MVS Bundle DM Std/800TSNB Reader Details. Figure 2. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. The analyzed results are. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. . …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. #3-000-510)Page 154: Ordering Parts And Accessories. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Two different agonists, acetylcholine and carbachol, were tested. A control well was set up to verify. A ten-fold serial dilution of purified luciferase in PBS with 0. Find regional support. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. or use the modified protocol below for the generation of NPCs (see Note 1). We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Analyst, M5, Flex 3, and SpectraMax L readers work differently. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. x. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. A = Log 10 (I 0 /I). Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Molecular Devices makes. SpectraMax M2 and M2e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. With optimized reagents, option to operate the. plates < 100 amol (384-well) 1 0200-7019. Features. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 6-well tissue-culture treated (TC) plate, sterile. VMax . Description. The system provides automation for up to 50 microplates for easy walkaway automation. 40266. Cell viability는 세포막 Integrity 또는. x. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 384-Well Fully Skirted Plates, Barcoded, including Armadillo 384-Well PCR plates. Dimensions 128 x 86 x 15 mm. Round well design with a clear flat bottom 2. (40 µl maximum) Clear, white, or black. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. Plate readers, also known as. All stand-alone instrument functions can be controlled using the software. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. X. Versa Max. After reading the ELISA plate on both readers, each standard curve was nearly identical. The fluorescent intensity of each well was measured by using a fluorescence plate reader (SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA, USA) at 588-nm excitation and 633-nm emission. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. 384-well format. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modefor the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Microplate format. Temperature control: 4°C above ambient to 45°C. x. Compare specifications and request a quote from leading suppliers. 25mm: Plate width: 85. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. MultiWash+ Microplate Washer. 7. Shaking: Linear and Orbital. 003 fmol 0. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. Microplate Formats: 6, 12, 24, 48, 96, 384. If you have an xml plate object, then. An industry-renowned instrument for monitoring GPCRs and ion channels. Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. ". Plate formats. for ELISAs and most cell-based assays. * Each reagent vial (Component A) is sufficient for 1 plate (96-, 384-, 1536-well). 5–25 µl. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wave-length range (spectral scan). • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Round well design with a solid black flat bottom2. 100 fmol luciferase per well. The FilterMax F3 can read 96-well and 384-well plates. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. Get Quote . Includes PC and software. 5 mP 1536–2. x. Infinite possibilities. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Temperature control. Abs). 8mm distance between well bottoms to plate bottom 50 µL total well volume, with recommended working volume of 5-40 µL High base plate design is compatible with automation and for bar coding Validated by Molecular Devices for the FLIPR reader. efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6 to 384 wells, the SpectraMax iD3 reader is the complete solution. 384-WELL SBS STERILE MICROPLATE. , San Diego, CA, USA) such that the. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. ZERO BIAS - scores, article reviews, protocol conditions and more. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 384 well plate (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. 20 attomol ATP per well. ©2010 Molecular Devices, Inc. Devices PathCheck® Sensor—the only patented technologySpectraMax ® M5 Multi-Mode Microplate Reader (Molecular Devices) Gemini EM Microplate Reader (Molecular Devices). Microplates, cuvettes (via adapter) Detectors. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent. 2. Figure 3. Industry standard disposable (choose from recommended list). The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. 1. The FLEXstation II 384 (Molecular Devices, Inc. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. EMax. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. It features a modular design that allows users to add or change detection cartridges according to their needs. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. both 96- and 384-well plates and comes with four wash/rinse bottles and one waste. 20 attomol ATP per well. Suggested plates are black. By measuring optical pathlength, it. Molecular Devices has provided scientists with tools to expand the boundaries of their research. Extended linear dynamic range. 1 fmol luciferase per well. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The microplate was then placed in a SpectraMax. MultiWash™+ Microplate Washer benefits include, walk-up usability, 4 wash and rinse. Time-resolved phosphorescence. Ensure data accuracy of your reader. by a photomultiplier tube (PMT) or charge-coupled device (CCD) present within the plate reader. Alpha 1536 HTS 96, 384, and . Key benefits • Assess cell confluence objectively and quantitatively. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells. 304 signal level saturation. Molecular Devices has developed the FLIPR Membrane Potential Assay Kits to maximize. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. A thermal cycling reaction was immediately initiated. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. x. 96-1536. 76 ± 0. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Get Quote . SpectraMax® Plus 384 provides ultrafast, full spectral range. VIS absorbance detection. Microplate readers are commonly used in life science laboratories. All stand-alone instrument functions can be controlled using the software. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. 100 fmol luciferase per well. Read the microplate. Fluorescent reading data were analyzed by non-linear fitting (GraphPad Prism, GraphPad Software Inc. Select Start>Control panel>Device Manager. Validation made easy -The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 19 H mm that meet microplate industry standards. White plates reflect the light, enabling even low levels of Luminescence to be detected. Two different agonists, acetylcholine and carbachol, were tested. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. #PLUS 384) UV-transparent 96-well microplates (Corning cat. Fluid dispensing speed. 0 and dispense 10 μL to all wells of the 384-well plate(s) (see Note 13). Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Outer wells aligned for proper viewing with FLIPR. #ID5) HTRF Detection System (Molecular Devices cat. x. Turn the. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. It also integrates with the. x. Values are averages from 5 clean plates, each filled with 200 µL water/well. Category 2 and 3 microplate readers use gradient heating as well. 00. 305 reference level saturation. 190. Overview. Streamline your workflow with comparative confluence assays to identify and verify gene edits. 046 to 0. 더 많은. T = I/I 0 and %T = 100 (T). Microplate readers provide the ideal measurement platform for the high-throughput. That explains the lower values on the LmaxII384 plate reader. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Dimensions: 18. No accessories are needed and SoftMax® Pro Software. M2. 100 fmol 384-well (75 µL) 0. Uncontrolled Copy for Training and Reference Purposes Only . 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Synergy NEO multi-mode plate reader: BioTek: n/a: EL406 plate washer and bulk dispenser with wide-bore tip 10 μL cassette: BioTek: n/a:. 4 decades . Select Update Driver. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. * Note: Programming the washer with a final aspirate height of 4. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. • 11/10 • Printed in USA • PN: 0120-1525. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 020 fmol 0. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. 0. flat meniscus), but is approx 15 mL. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. M5. 5in (46. Minimum volume and maximum throughput with 96- or. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. EMax. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. . tools to expand the boundaries of their research. 0 mP 96-well. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. Figure 6. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Service parts for these models are not being produced and are no longer in stock. #4TI-0655) Microplates. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Total volume (except deep. 3. Suitable for use in BMG lab tech, Molecular Devices, Promega Glomax and other plate readers; Suitable for adhesive and heat sealing: our Moisture Barrier Seal 384. x. 96- and 384-well microplates. This kit is designed for customers who. 2. Figure 1. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). 2, prime the machine with CellTiter-Glo ® 2. High base plate design is compatible with automated handling (gripping) and bar coding. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. Selects either 96-well or 384-well plate for a stand-alone read. QuickSync. 1 OD at 230 nm. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. $542. 039. The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. Select the Driver tab. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The MultiWash+ Microplate Washer is an automated washer that is configurable for both 96- and 384- well plates. 012 fmol 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Test up to 3. Add 100 µl (96-well plate) or 25 µl (384-well plate) of cell suspension to each well of poly-D-lysine coated plates. Contact our support team with your questions regarding Molecular Devices products and applications. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Technical specifications. the iD5 reader’s filter system. Description: The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. Industry standard disposable (choose from recommended list). The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. 8 mm distance between well bottoms to plate bottom. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Reconstitution of reagents. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. The equation that allows one to calculate absorbance from % transmittance is. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. x. Microplate Spectrophotometer. Bioz Stars score: 86/100, based on 1 PubMed. Embla washers are durable and easy to maintain. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. 340 PC384. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. In general microplate washers are designed to leave only 2 microliters of residue per well and operate with an accuracy of 3% CV per well and a uniformity of 4% CV across a plate. Packed 25 plates per sleeve, 2 sleeves per case. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 190. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. 312 gain calibration failed. UV absorbance detection. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. ) 0. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Plus 384. 5 pM 2. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Microplates, cuvettes (via adapter) Detectors. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 190. Rating: 3. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. plates < 100 amol (384-well) 1 0200-7018. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. It. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Ideal for miniaturized 384 well fluorescent. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. VIS absorbance detection. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. The GenePix® Microarray Systems are based on 25 years of expertise in low-noise signal amplification and optical design. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. Temperature Control: Ambient +5C up to 42C. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV.